Part:BBa_K2057006:Design
Expression of gfp under the regulation of promoter BtI-BtII
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1037
Design Notes
As promoter BtI-BtII is not available in the biobrick we amplify from the cry1Ac gene
Source
The promoter BtI-BtII is located on a megaplasmid of Bacillus thuringiensis and control the expression of cry1Ac. gfp is obtained from the biobrick BBa_E0040
References
(ii). Von Tersch MA, Robbins HL, Jany CS, Johnson TB (1991) Insecticidal toxins from Bacillus thuringiensis subsp. kenyae: gene cloning and characterization and comparison with B. thuringiensis subsp. kurstaki CryIA(c) toxins Appl. Environ. Microbiol. 57 (2): 349-358. (ii). Sedlak M1, Walter T, Aronson A (2000) Regulation by overlapping promoters of the rate of synthesis and deposition into crystalline inclusions of Bacillus thuringiensis delta-endotoxins. J Bacteriol. 182(3):734-41.